2EUH

HOLO FORM OF A NADP DEPENDENT ALDEHYDE DEHYDROGENASE COMPLEX WITH NADP+

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Spacegroup name	P 21 21 2
Unit cell lengths	144.800, 154.800, 114.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.235
Rwork	0.235
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1euh
RMSD bond length	0.008
RMSD bond angle	25.380 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.600
High resolution limit [Å]	2.600
Rmerge	0.087 *	0.315 *
Number of reflections	65491
Completeness [%]	82.6
Redundancy	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	drop	NADP
4	1	reservoir	ammonium sulfate	2.0 (M)
5	1	reservoir	PEG400	2 (%(w/v))
6	1	reservoir	HEPES	0.1 (M)