2EUD

Structures of Yeast Ribonucleotide Reductase I complexed with Ligands and Subunit Peptides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	107.578, 117.216, 63.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.21
Rwork	0.204
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	35816
<I/σ(I)>	17.6	2.6
Completeness [%]	97.5	87.4
Redundancy	4.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	298	0.1 M sodium acetate, 20-25% PEG3350, 0.2 M ammonium sulfate, pH 6.5, EVAPORATION, temperature 298K