2EUC
Crystal structure of YfmB from Bacillus subtilis. NESG TARGET SR324
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9789, 0.9795, 0.96804 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.543, 67.637, 78.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.270 - 2.500 |
| R-factor | 0.274 |
| Rwork | 0.274 |
| R-free | 0.32800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.258 |
| Number of reflections | 22474 | |
| <I/σ(I)> | 35.107 | 1.76 |
| Completeness [%] | 95.5 | 83.8 |
| Redundancy | 5.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | VAPOR DIFFUSION, HANGING DROP, pH 7.5, temperature 293K |
