2EU9
Crystal Structure of CLK3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.727, 45.063, 84.006 |
| Unit cell angles | 90.00, 115.23, 90.00 |
Refinement procedure
| Resolution | 30.710 - 1.530 |
| R-factor | 0.17146 |
| Rwork | 0.171 |
| R-free | 0.20098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.743 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.710 | 1.650 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.058 | 0.378 |
| Number of reflections | 55697 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 282 | 27% PEG 3350, 30 mM Ammonium acetate, 100 mM Bis Tris pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 282K |
