2EU1
Crystal structure of the chaperonin GroEL-E461K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 267.695, 290.641, 247.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.290 |
R-factor | 0.276 |
Rwork | 0.276 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oel |
RMSD bond length | 0.011 |
RMSD bond angle | 1.329 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.750 |
High resolution limit [Å] | 3.290 |
Number of reflections | 123350 |
Completeness [%] | 85.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | Reservoir solution: 43% (v/v) MPD, 100mM Imidazole, 170mM MgCl2.6H2O, dissolved in the ratio 1:1.5 with 22.5mg/ml protein, 50mM TRIS-HCL, 10mM MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |