2ETN
Crystal structure of Thermus aquaticus Gfh1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2003-06-30 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 191.200, 76.400, 53.100 |
Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.300 |
R-factor | 0.25742 |
Rwork | 0.250 |
R-free | 0.32464 |
Structure solution method | MAD |
RMSD bond length | 0.033 |
RMSD bond angle | 3.009 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 12359 | |
<I/σ(I)> | 18.5 | 2.2 |
Completeness [%] | 99.2 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 50mM sodium Cacodylate pH 6.0, 10 mM magnesium sulfate, 1.9 M lithium sulfate 1% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 50mM sodium Cacodylate pH 6.0, 10 mM magnesium sulfate, 1.9 M lithium sulfate 1% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |