2ETL
Crystal Structure of Ubiquitin Carboxy-terminal Hydrolase L1 (UCH-L1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2005-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98397 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 110.097, 110.097, 79.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 2.400 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on UCH-L3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 | 0.773 |
| Number of reflections | 34753 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 14.1 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2.4 M Ammonium Sulfate, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
