2ETJ

Crystal structure of Ribonuclease HII (EC 3.1.26.4) (RNase HII) (tm0915) from THERMOTOGA MARITIMA at 1.74 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9797, 1.0000
Spacegroup name	P 31
Unit cell lengths	51.821, 51.821, 76.293
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.150 - 1.740
Rwork	0.179
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.572
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.150	18.150	1.790
High resolution limit [Å]	1.740	7.780	1.740
Rmerge	0.069	0.048	0.469
Number of reflections	23391
<I/σ(I)>	7.1	14	1.5
Completeness [%]	99.4
Redundancy	2.8	2.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5.8	277	0.2M MgCl2, 20.0% PEG-3350, No Buffer , pH 5.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K