2ETC
Crystal structure of the ankyrin repeat domain of TRPV2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97922 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.514, 95.862, 164.504 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.21499 |
Rwork | 0.210 |
R-free | 0.26184 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eta |
RMSD bond length | 0.006 |
RMSD bond angle | 0.961 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.140 | 0.493 |
Number of reflections | 11944 | |
<I/σ(I)> | 17.4 | 5.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.33mM protein, 9% PEG8000, 0.1M sodium citrate, pH 5.0, 5-7% xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |