2ET6
(3R)-Hydroxyacyl-CoA Dehydrogenase Domain of Candida tropicalis Peroxisomal Multifunctional Enzyme Type 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.895, 78.340, 95.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.220 |
| R-factor | 0.19784 |
| Rwork | 0.195 |
| R-free | 0.25183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IGZ6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.296 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.360 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Number of reflections | 27948 | |
| <I/σ(I)> | 12.56 | 4.74 |
| Completeness [%] | 98.7 | 96.4 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 30% PEG 4000, 0.1 M sodium acetate pH 4.2, 0.2 M ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
