2ET1
Oxalate oxidase in complex with substrate analogue glycolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 96.302, 96.302, 108.133 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.520 - 1.600 |
| R-factor | 0.17809 |
| Rwork | 0.177 |
| R-free | 0.20600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Oxalate oxidase (pdb entry 1fi2) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.391 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.025 | 0.047 |
| Number of reflections | 24460 | |
| <I/σ(I)> | 40 | 26 |
| Completeness [%] | 97.6 | 98.4 |
| Redundancy | 15 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 2.3M ammonium sulfate, 5% 2-propanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
