2ESP
Human ubiquitin-conjugating enzyme (E2) UbcH5b mutant Ile88Ala
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97897 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.877, 49.224, 62.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.580 - 1.520 |
| R-factor | 0.18663 |
| Rwork | 0.184 |
| R-free | 0.23342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2esk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.045 | 0.522 |
| Number of reflections | 22996 | |
| <I/σ(I)> | 31.6 | 2.2 |
| Completeness [%] | 98.7 | 91 |
| Redundancy | 4.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | NaCl, PEG 6000, Sodium Acetate, temperature 294K, pH 4.5, VAPOR DIFFUSION, HANGING DROP |
