2ESO
Human Ubiquitin-Conjugating Enzyme (E2) UbcH5b mutant Ile37Ala
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-15 |
Detector | SBC-3 |
Wavelength(s) | 0.97897 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.654, 49.429, 63.037 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.900 - 1.500 |
R-factor | 0.17938 |
Rwork | 0.177 |
R-free | 0.21913 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2esk |
RMSD bond length | 0.020 |
RMSD bond angle | 2.067 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.058 | 0.796 |
Number of reflections | 24280 | |
<I/σ(I)> | 42.6 | 2.6 |
Completeness [%] | 99.0 | |
Redundancy | 9.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | NaCl, PEG 6000, Sodium Acetate, temperature 294K, pH 4.5, VAPOR DIFFUSION, HANGING DROP |