2ESK
Human Ubiquitin-Conjugating Enzyme (E2) UbcH5b, wild-type
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-15 |
Detector | SBC-3 |
Wavelength(s) | 0.97897 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.597, 49.372, 62.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.720 - 1.360 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qcq |
RMSD bond length | 0.017 |
RMSD bond angle | 1.872 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.410 |
High resolution limit [Å] | 1.360 | 2.930 | 1.360 |
Rmerge | 0.041 | 0.021 | 0.496 |
Number of reflections | 31496 | ||
Completeness [%] | 97.2 | ||
Redundancy | 7 | 7.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 294 | NaCl, PEG 6000, Sodium Acetate, temperature 294K, pH 4.5, VAPOR DIFFUSION, HANGING DROP |