2ESH
Crystal Structure of Conserved Protein of Unknown Function TM0937- a Potential Transcriptional Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-11 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9794807 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.648, 50.648, 114.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.850 - 2.300 |
| R-factor | 0.22786 |
| Rwork | 0.226 |
| R-free | 0.27034 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.973 |
| Number of reflections | 7620 | |
| <I/σ(I)> | 23.13 | 2.93 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.9 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 0.4M CaCL2, 20% PEG3350, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
