2ERQ

Crystal structure of vascular apoptosis-inducing protein-1(tetragonal crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2004-07-04
Detector	RIGAKU
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	93.940, 93.940, 244.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.23
Rwork	0.229
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ero
RMSD bond length	0.008
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.084	0.380
Number of reflections	38868
<I/σ(I)>	18.7
Completeness [%]	99.7	99.6
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	15% PEG8000, 100mM sodium/cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K