2ERQ
Crystal structure of vascular apoptosis-inducing protein-1(tetragonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-04 |
| Detector | RIGAKU |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.940, 93.940, 244.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ero |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.084 | 0.380 |
| Number of reflections | 38868 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15% PEG8000, 100mM sodium/cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
