2ERK

PHOSPHORYLATED MAP KINASE ERK2

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	110
Detector technology	CCD
Collection date	1996-03-10
Spacegroup name	P 41 21 2
Unit cell lengths	92.500, 92.500, 103.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.192
Rwork	0.192
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ERK
RMSD bond length	0.010 *
RMSD bond angle	1.344 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.079 *	0.201 *
Total number of observations	105306 *
Number of reflections	16537	1070 *
<I/σ(I)>	27	8
Completeness [%]	92.1	60.3 *
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		20% PEG 4000, 200 MM (NH4)2SO4, 100 MM MES PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	MES	100 (mM)	pH6.5