2ERK
PHOSPHORYLATED MAP KINASE ERK2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1996-03-10 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.500, 92.500, 103.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ERK |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.344 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 * | 0.201 * |
| Total number of observations | 105306 * | |
| Number of reflections | 16537 | 1070 * |
| <I/σ(I)> | 27 | 8 |
| Completeness [%] | 92.1 | 60.3 * |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20% PEG 4000, 200 MM (NH4)2SO4, 100 MM MES PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | MES | 100 (mM) | pH6.5 |
