2ERG
Crystal Structure of Leu3 DNA-binding domain with a single H50C mutation complexed with a 15mer DNA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2000-06-08 |
Wavelength(s) | 0.9213 |
Spacegroup name | P 62 |
Unit cell lengths | 66.768, 66.768, 122.745 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.090 - 3.150 |
R-factor | 0.273 |
Rwork | 0.273 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ere |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 5419 | |
<I/σ(I)> | 3.7 | 2.9 |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50 mM Mes, 3% v/v isopropanol, 20 mM Mg(OAc)2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Mes | ||
2 | 1 | 1 | isopropanol | ||
3 | 1 | 1 | Mg(OAc)2 | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Mes | ||
6 | 1 | 2 | Mg(OAc)2 | ||
7 | 1 | 2 | H2O |