2EQA
Crystal Structure of the hypothetical Sua5 protein from Sulfolobus tokodaii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97915, 0.9, 0.97973 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 85.391, 120.843, 67.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.090 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.139 |
| Number of reflections | 32701 | |
| <I/σ(I)> | 52.47 | 12.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1M Sodium Acetate, 4.0M Sodium Nitrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
