2EQ9
Crystal structure of lipoamide dehydrogenase from thermus thermophilus HB8 with psbdb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-03-08 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.781, 88.877, 144.149 |
| Unit cell angles | 89.26, 87.01, 70.84 |
Refinement procedure
| Resolution | 43.620 - 2.090 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eq6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.033 | 0.256 |
| Number of reflections | 238181 | |
| <I/σ(I)> | 18.3 | 1.7 |
| Completeness [%] | 96.8 | 87.4 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 30%(w/v) PEG MME 5000, 200mM ammonium sulfate, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
