2EPN
N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.574, 112.332, 103.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.020 - 1.610 |
| R-factor | 0.16912 |
| Rwork | 0.167 |
| R-free | 0.20186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2epk |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.330 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.020 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.064 | 0.500 |
| Number of reflections | 161033 | |
| <I/σ(I)> | 20.9 | 3.51 |
| Completeness [%] | 99.3 | 94.37 |
| Redundancy | 9.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM HEPES, 2M ammonium sulphate, 0.5%(v/v) PEG400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
