2EPM
N-acetyl-B-D-glucoasminidase (GCNA) from Stretococcus gordonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 122.964, 124.746, 147.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.950 - 2.040 |
| R-factor | 0.20091 |
| Rwork | 0.199 |
| R-free | 0.23699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2epl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.245 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.950 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.064 | 0.350 |
| Number of reflections | 71742 | |
| <I/σ(I)> | 18.9 | 3.74 |
| Completeness [%] | 99.3 | 94.31 |
| Redundancy | 8.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 100mM Tris, 1.9M ammonium sulphate, 0.5%(v/v) PEG400, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
