2EPK
N-acetyl-B-D-glucosaminidase (GCNA) from Streptococcus gordonii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97928, 0.97945, 0.94929 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 123.895, 124.509, 148.536 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.970 - 1.850 |
| R-factor | 0.21605 |
| Rwork | 0.215 |
| R-free | 0.23470 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.970 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 97524 | |
| <I/σ(I)> | 13.4 | 3.24 |
| Completeness [%] | 99.6 | 95.58 |
| Redundancy | 7.4 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM HEPES, 2M ammonium sulphate, 0.5 %(v/v) PEG400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
