2EPI
Crystal Structure pf hypothetical protein MJ1052 from Methanocaldococcus jannascii (Form 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-09 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.780, 75.747, 54.991 |
Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lxn |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.061 | 0.376 |
Number of reflections | 41290 | |
<I/σ(I)> | 11.9 | 2.24 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.9 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7 | 295 | 30%(v/v) Jeffamine M-600, 0.1M HEPES, pH 7.0, microbatch, temperature 295K |