2EPE

Crystal structure analysis of Hen egg white lysozyme grown by capillary method

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	79.243, 79.243, 37.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.530 - 2.500
R-factor	0.16681
Rwork	0.165
R-free	0.21131
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.037
RMSD bond angle	2.441
Data reduction software	AUTOMAR
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.530	39.530
High resolution limit [Å]	2.500	2.500
Rmerge		0.092
Number of reflections	4332
<I/σ(I)>		5.9
Completeness [%]		98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	capillary method	4.7	298	0.04m NAOAC/HOAC buffer, pH 4.7, capillary method, temperature 298.0K