2EP5
Structural study of Project ID ST1242 from Sulfolobus tokodaii strain7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-06 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 70.157, 73.986, 91.552 |
Unit cell angles | 68.10, 68.39, 86.04 |
Refinement procedure
Resolution | 29.270 - 2.400 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.063 | 0.193 |
Number of reflections | 58141 | |
<I/σ(I)> | 11.4 | 4.18 |
Completeness [%] | 94.8 | 94.4 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 291 | 8w/v(%) PEG 4K, 0.1M Acet, pH 4.6, MICROBATCH, temperature 291K |