2EO0
Crystal Structure of Holliday Junction Resolvase ST1444
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-01-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.661, 49.326, 69.894 |
Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
Resolution | 25.870 - 2.400 |
R-factor | 0.2325 |
Rwork | 0.232 |
R-free | 0.31200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hh1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.485 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.064 | 0.508 |
Number of reflections | 11787 | |
<I/σ(I)> | 14.4 | 3.75 |
Completeness [%] | 99.6 | 98 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 1.4M tri-Sodium Citrate dehydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |