2EMQ
Hypothetical Conserved Protein (GK1048) from Geobacillus Kaustophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-16 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9794 |
Spacegroup name | P 65 |
Unit cell lengths | 56.194, 56.194, 211.224 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yav |
RMSD bond length | 0.010 |
RMSD bond angle | 1.322 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.088 | 0.526 |
Number of reflections | 12961 | |
<I/σ(I)> | 18 | 12.77 |
Completeness [%] | 99.8 | 100 |
Redundancy | 16.4 | 16.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2M Li2SO4, 0.1 M Tris, 1M Na tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |