2EMO

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1996-07-15
Detector	SIEMENS HI-STAR
Spacegroup name	P 61 2 2
Unit cell lengths	88.700, 88.700, 130.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.203
Rwork	0.203
R-free	0.34300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.091 *	0.306 *
Number of reflections	11067
<I/σ(I)>	16.7	2
Completeness [%]	78.1	50 *
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	20 *	protein solution is mixed in a 1:1 ratio with well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	21 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	1.95 (M)
4	1	reservoir	Tris-HCl	100 (mM)