2EMD
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-25 |
| Detector | MACSCIENCE |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 175.600, 175.600, 175.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.014 |
| RMSD bond angle | 28.490 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 * | 0.231 * |
| Number of reflections | 25953 | |
| <I/σ(I)> | 15.6 | 2.9 |
| Completeness [%] | 82.8 | 46.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 21 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) |
