2ELG
The rare crystallographic structure of d(CGCGCG)2: The natural spermidine molecule bound to the minor groove of left-handed Z-DNA d(CGCGCG)2 at 10 degree celsius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2005-12-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 17.847, 30.988, 44.022 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 1.000 |
R-factor | 0.244 |
Rwork | 0.232 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dj6 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MERLOT |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.047 | 0.047 |
Number of reflections | 11287 | |
<I/σ(I)> | 20.7 | 20.7 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 10mM Sodium cacodilate, 1M Magnesium chloride, 100mM spermidine trichloride, 8%MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium cacodilate | ||
2 | 1 | 1 | Magnesium chloride | ||
3 | 1 | 1 | spermidine trichloride | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | Sodium cacodilate | ||
7 | 1 | 2 | Magnesium chloride | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | HOH |