2EKZ
Structural study of Project ID TTHB049 from Thermus thermophilus HB8 (L52M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-02-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.910, 91.976, 111.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.640 - 1.850 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.073 | 0.436 |
| Number of reflections | 37261 | |
| <I/σ(I)> | 10.3 | 4.5 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 9.2 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.3 | 291 | 2.75M Na Chloride, pH 8.27 0.1M Tris, pH 8.3, MICROBATCH, temperature 291K |
