2EKL
Structure of ST1218 protein from Sulfolobus tokodaii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.065, 59.798, 60.728 |
| Unit cell angles | 90.00, 114.30, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.770 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g76 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.036 | |
| Number of reflections | 33238 | |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.9 | 98 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 0.1M NH(2)SO(4), 20% PEG 4K, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
