2EKD
Structural study of Project ID PH0250 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-19 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.97907 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.471, 158.026, 163.347 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 0.148 |
| Number of reflections | 54164 | |
| <I/σ(I)> | 20.8 | 12.16 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.3 | 291 | 40%(v/v) 1,2-propanediol, Acetate pH 4.5, 0.05M Ca(OAc)2, pH 5.3, MICROBATCH, temperature 291K |
