2EKC
Structural study of Project ID aq_1548 from Aquifex aeolicus VF5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-11 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.854, 72.985, 64.942 |
Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
Resolution | 29.670 - 2.000 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 | 0.228 |
Number of reflections | 36575 | |
<I/σ(I)> | 10 | 4.17 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.1 | 291 | 30v/v(%) PEG 200, 0.1M MES, 5w/v(%) PEG 3K, pH 6.1, MICROBATCH, temperature 291K |