2EKA
Structural study of Project ID PH0725 from Pyrococcus horikoshii OT3 (L202M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-10 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 105.433, 105.433, 139.454 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.820 - 2.300 |
R-factor | 0.212 |
Rwork | 0.210 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.047 | 0.297 |
Number of reflections | 35451 | |
<I/σ(I)> | 15.2 | 6.83 |
Completeness [%] | 99.7 | 100 |
Redundancy | 13.6 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 291 | 3.85M Na Formate ,0.1M Acetate, pH 5.5, MICROBATCH, temperature 291K |