2EK6
Crystal structure of Human RNA-Binding Protein 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 6 |
| Unit cell lengths | 107.020, 107.020, 58.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.700 - 2.380 |
| R-factor | 0.167 |
| Rwork | 0.167 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 2EK1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.727 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.148 | 0.462 |
| Number of reflections | 15528 | |
| <I/σ(I)> | 8.7 | 10.65 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1M MES-NaOH, 20% PEG 4000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
