2EJX

Crystal structure of the hypothetical protein STK_08120 from Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	54.887, 70.446, 35.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.790
R-factor	0.22246
Rwork	0.220
R-free	0.26171
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.612
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.790	1.790
Number of reflections	13524
<I/σ(I)>	43.9	10.8
Completeness [%]	97.8	96.6
Redundancy	5.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	15% PEG4000, 20% isopropanol, 100mM MES, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	15% PEG4000, 20% isopropanol, 100mM MES, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K