2EJN
Structural characterization of the tetrameric form of the major cat allergen fel D 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.087 |
Spacegroup name | P 1 |
Unit cell lengths | 38.588, 42.996, 48.906 |
Unit cell angles | 70.80, 80.56, 81.53 |
Refinement procedure
Resolution | 36.510 - 1.640 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1puo |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.510 |
High resolution limit [Å] | 1.640 |
Number of reflections | 31695 |
Completeness [%] | 92.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 22.5% PEG3350, 0.5M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |