2EJJ
Mutant K129M structure of PH0725 from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 104.773, 104.773, 137.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 2.100 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wng |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.491 |
| Number of reflections | 45211 | |
| <I/σ(I)> | 12.3 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbach | 7 | 295 | 1.87M Na Malonic acid, 0.1M MES, 1mM DTT, pH 7.0, Microbach, temperature 295K |
