2EJG
Crystal Structure Of The Biotin Protein Ligase (Mutation R48A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.572, 63.487, 74.715 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 38.930 - 2.710 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2DZC AND 2D5D |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 2.800 |
| High resolution limit [Å] | 2.710 | 2.700 |
| Rmerge | 0.107 | 0.320 |
| Number of reflections | 16349 | |
| <I/σ(I)> | 6.7 | 2.03 |
| Completeness [%] | 91.1 | 86.6 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.96 | 295 | 10.5w/v(%) PEG 20000, 0.1M Acet, NaOH, 2.5M Biotin, 2.5M ATP, pH 4.96, microbatch, temperature 295K |
