2EJ5
Crystal structure of GK2038 protein (enoyl-CoA hydratase subunit II) from Geobacillus kaustophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-12 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97894 |
Spacegroup name | P 21 3 |
Unit cell lengths | 117.008, 117.008, 117.008 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.450 - 2.000 |
R-factor | 0.19434 |
Rwork | 0.192 |
R-free | 0.24229 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.597 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.087 | 0.266 |
Number of reflections | 36275 | |
<I/σ(I)> | 19.5 | 12.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20.9 | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG3350, 0.1M magnesium formate, pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG3350, 0.1M magnesium formate, pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG3350, 0.1M magnesium formate, pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |