2EIV
Crystal Structure of the arginase from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-10-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 159.977, 139.893, 84.371 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.910 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ef4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.449 |
| Number of reflections | 80781 | |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 8% PEG MME2000, 0.05M Bis Tris Propane pH7.0, 0.005M Ammonium Acetate, 0.1M Guanidine HCL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
