2EIF
Eukaryotic translation initiation factor 5A from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.483, 40.198, 48.580 |
Unit cell angles | 90.00, 124.29, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eif |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS (0.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.233 |
Number of reflections | 11659 | |
<I/σ(I)> | 16.2 | 3.7 |
Completeness [%] | 96.7 | 91.2 |
Redundancy | 1.98 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 | 4 * | 0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000 |
1 | Vapor diffusion * | 8.5 | 4 * | 0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000 |
1 | Vapor diffusion * | 8.5 | 4 * | 0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.1 (M) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | PEG4000 | 30 (%) |