2EID

Galactose Oxidase W290G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.864
Spacegroup name	P 65 2 2
Unit cell lengths	89.884, 89.884, 415.325
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.200
Rwork	0.192
R-free	0.22600
Structure solution method	FOURIER SYNTHESIS
RMSD bond angle	0.013
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.200
Rmerge	0.070
Number of reflections	12672
Completeness [%]	64.6
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	1-2M ammonium sulphate, 0.1 sodium acetate pH 4-5, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K