2EIC

Crystal Structure of Galactose Oxidase mutant W290F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.864
Spacegroup name	C 1 2 1
Unit cell lengths	97.770, 88.890, 86.190
Unit cell angles	90.00, 117.90, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.177
R-free	0.23300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1gog
RMSD bond length	0.010
RMSD bond angle	1.550
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Rmerge	0.084
Number of reflections	14432
Completeness [%]	89.3
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	1-2M Ammonium Sulphate, 0.1M sodium acetate pH 4-5, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K