2EI9
Crystal structure of R1Bm endonuclease domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 141.303, 141.303, 37.508 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
Rwork | 0.195 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wdu |
RMSD bond length | 0.005 |
RMSD bond angle | 1.304 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.366 |
Number of reflections | 29191 | |
<I/σ(I)> | 6.8 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.3 | 19.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 283 | 2.4M sodium acetate, 10mM ammonium sulfate, 2% Jeffamine M600, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 283K |