2EI4
Trimeric complex of archaerhodopsin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 98.160, 98.160, 56.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| Rwork | 0.188 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vgo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.108 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.098 | 0.420 |
| Number of reflections | 18116 | |
| <I/σ(I)> | 6.2 | 3.8 |
| Completeness [%] | 98.4 | 99.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 2.8M ammonium sulfate, 0.1M HEPES, 0.32% nonylglucoside, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
