2EI2
Crystal Structure Analysis of the 1,2-dihydroxynaphthalene dioxygenase from Pseudomonas sp. stain C18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-07 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.99 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 118.390, 118.390, 120.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.970 - 1.690 |
| R-factor | 0.1626 |
| Rwork | 0.161 |
| R-free | 0.18629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1han |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.970 | |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 46344 | |
| Completeness [%] | 97.1 | 97.6 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 1.8M NaCl, 0.1M MgCl2, 0.1M Hepes, naerobic crystallization, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
