2EHB
The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.137, 57.387, 141.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.890 - 2.100 |
R-factor | 0.2168 |
Rwork | 0.215 |
R-free | 0.25932 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Structure of SOS3 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.388 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.900 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.350 |
Number of reflections | 25141 | |
<I/σ(I)> | 9.7 | 2.6 |
Completeness [%] | 99.3 | 92.7 |
Redundancy | 4.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | drops: 10mg/ml SOS3:C-SOS2, reservoir solution: 20%(w/v) PEG4000, 0.1M Tris-HCl pH 7.5, 0.2M Lithium Sulfate, 3%(v/v) Ethylene Glycol; 1M Guanidine HCl in a ratio of 1:1:0.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |